TNFRSF21 Protein (AA 42-349) (His tag)

Catalog No. ABIN7274497

This Recombinant TNFRSF21 protein is expressed in HEK-293 Cells.

Quick Overview for TNFRSF21 Protein (AA 42-349) (His tag) (ABIN7274497)

Target: TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 42-349

Purification tag / Conjugate: This TNFRSF21 protein is labelled with His tag.

Purpose: Cynomolgus DR6/TNFRSF21 Protein

Sequence: Gln42-His349

Characteristics: Recombinant Cynomolgus DR6/TNFRSF21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln42-His349.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus DR6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DR6 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.,Cynomolgus DR6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus DR6 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))

Alternative Name: DR6

Background: Beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. DR6 is broadly expressed by developing neurons, and is required for normal cell body death and axonal pruning both in vivo and after trophic-factor deprivation in vitro.DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation.

Molecular Weight: 34.50 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5VIJ8

Pathways: Regulation of Lipid Metabolism by PPARalpha