EGF Protein (AA 971-1023)

Catalog No. ABIN7274522

Recombinant EGF protein expressed in Escherichia coli (E. coli).

Quick Overview for EGF Protein (AA 971-1023) (ABIN7274522)

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 971-1023

Purpose: Human EGF Protein

Sequence: Asn971-Arg1023

Characteristics: Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.2 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Molecular Weight: 5.6 kDa same as Tris-Bis PAGE result.

UniProt: P01133-1

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation