EPH Receptor B2 Protein (EPHB2) (AA 19-542) (His tag)
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- Target See all EPH Receptor B2 (EPHB2) Proteins
- EPH Receptor B2 (EPHB2)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-542
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EPH Receptor B2 protein is labelled with His tag.
- Purpose
- Cynomolgus EPHB2 Protein
- Sequence
- Val19-Pro542
- Characteristics
- Recombinant Cynomolgus EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro542.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product EPHB2 Protein
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EPH Receptor B2 (EPHB2) (AA 19-543) protein (His tag)
EPHB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B2 (EPHB2) (AA 19-543) protein (His tag)EPHB2 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B2 (EPHB2) (AA 570-987) (Active) protein (GST tag,His tag)EPHB2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
EPH Receptor B2 (EPHB2) (AA 19-543) protein (His tag)EPHB2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- EPH Receptor B2 (EPHB2)
- Alternative Name
- EPHB2 (EPHB2 Products)
- Background
- EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
- Molecular Weight
- 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A7N9CQH5
- Pathways
- RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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