EPH Receptor B3 Protein (EPHB3) (AA 34-559) (His tag)
Quick Overview for EPH Receptor B3 Protein (EPHB3) (AA 34-559) (His tag) (ABIN7274559)
Target
See all EPH Receptor B3 (EPHB3) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 34-559
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Purification tag / Conjugate
- This EPH Receptor B3 protein is labelled with His tag.
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Purpose
- Human EphB3 Protein
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Sequence
- Gly34-Leu559
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Characteristics
- Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly34-Leu559.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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EPH Receptor B3 (EPHB3) (AA 30-554) protein (His tag)
EPHB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B3 (EPHB3) (AA 1-998) protein (GST tag)EPHB3 Origin: Human Host: Wheat germ Recombinant WB, AA, AP, ELISA
EPH Receptor B3 (EPHB3) (Active) protein (His tag)EPHB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 94 % as determined by SDS-PAGE Active
EPH Receptor B3 (EPHB3) protein (DYKDDDDK Tag)EPHB3 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
EPH Receptor B3 (EPHB3) protein (Myc-DYKDDDDK Tag)EPHB3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
EPH Receptor B3 (EPHB3) protein (DYKDDDDK-His Tag)EPHB3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- EPH Receptor B3 (EPHB3)
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Alternative Name
- EphB3
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Background
- Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.
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Molecular Weight
- 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
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UniProt
- P54753
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Pathways
- RTK Signaling
Target
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