EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag)

Catalog No. ABIN7274560

This Recombinant EPH Receptor B2 protein is expressed in HEK-293 Cells.

Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag) (ABIN7274560)

Target: EPH Receptor B2 (EPHB2)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-543

Purification tag / Conjugate: This EPH Receptor B2 protein is labelled with His tag.

Purpose: Mouse EPHB2 Protein

Sequence: Val19-Leu543

Characteristics: Recombinant Mouse EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background: EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.

Molecular Weight: 59.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: P54763-3

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins