EPH Receptor A4 Protein (EPHA4) (AA 20-547) (His tag)

Catalog No. ABIN7274561

Recombinant EPH Receptor A4 protein expressed in HEK-293 Cells.

Quick Overview for EPH Receptor A4 Protein (EPHA4) (AA 20-547) (His tag) (ABIN7274561)

Target: EPH Receptor A4 (EPHA4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-547

Purification tag / Conjugate: This EPH Receptor A4 protein is labelled with His tag.

Purpose: Mouse EPHA4 Protein

Sequence: Val20-Thr547

Characteristics: Recombinant Mouse EPHA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Thr547.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse EPHA4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse EPHA4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EPHA4

Background: The expression and activation of EphA4 in the various cell types in a knee joint was upregulated upon an intraarticular injury. To determine if EphA4 signaling plays a role in osteoarthritis, we determined whether deficient EphA4 expression (in EphA4 knockout mice) or upregulation of the EphA4 signaling (with the EfnA4-fc treatment) would alter cellular functions of synoviocytes and articular chondrocytes. In synoviocytes, deficient EphA4 expression enhanced, whereas activation of the EphA4 signaling reduced, expression and secretion of key inflammatory cytokines and matrix metalloproteases.

Molecular Weight: 59.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.

UniProt: Q03137

Pathways: RTK Signaling