CD147 Protein (AA 22-205) (His tag)

Catalog No. ABIN7274567

Product Details

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Protein Type: Recombinant

Protein Characteristics: AA 22-205

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD147 protein is labelled with His tag.

Purpose: Human EMMPRIN/CD147 Protein

Sequence: Ala22-His205

Characteristics: Recombinant Human EMMPRIN/CD147 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala22-His205.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EMMPRIN, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: EMMPRIN (BSG Products)

Background: CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.

Molecular Weight: 21.2 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_940991

Pathways: S100 Proteins