CD147 Protein (AA 22-205) (His tag,AVI tag,Biotin)

Catalog No. ABIN5526649

Product Details

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Protein Type: Recombinant

Protein Characteristics: AA 22-205

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD147 protein is labelled with His tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 22-205

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 22.8 kDa. The protein migrates as 30-45 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >92 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: EMMPRIN (BSG Products)

Synonyms: EMMPRIN Protein, RPE7 Protein, 5F7 Protein, CD147 Protein, M6 Protein, OK Protein, TCSF Protein, AI115436 Protein, AI325119 Protein, HT-7 Protein, 5A11 Protein, CE9 Protein, HT7 Protein, Ox47R Protein, basignin Protein, wu:fb26a05 Protein, wu:fo98e12 Protein, bsg Protein, prso03 Protein, basigin (Ok blood group) Protein, basigin Protein, basigin (Ok blood group) S homeolog Protein, BSG Protein, Bsg Protein, bsg Protein, bsg.S Protein

Background: CD147 is also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.

Molecular Weight: 22.8 kDa

NCBI Accession: NP_940991

Pathways: S100 Proteins