Epiregulin Protein (EREG) (AA 56-101) (hFc Tag)

Catalog No. ABIN7274569

This Recombinant Epiregulin protein is expressed in HEK-293 Cells.

Quick Overview for Epiregulin Protein (EREG) (AA 56-101) (hFc Tag) (ABIN7274569)

Target: Epiregulin (EREG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 56-101

Purification tag / Conjugate: This Epiregulin protein is labelled with hFc Tag.

Purpose: Mouse EREG Protein

Sequence: Val56-Leu101

Characteristics: Recombinant Mouse EREG Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Val56-Leu101.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse EREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse EREG is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Epiregulin (EREG)

Alternative Name: EREG

Background: Epidermal growth factor receptor (EGFR) regulates many crucial cellular programs, with seven different activating ligands shaping cell signaling in distinct ways.EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. As a consequence, EREG or EPGN induce less stable EGFR dimers than EGF-making them partial agonists of EGFR dimerization. Unexpectedly, this weakened dimerization elicits more sustained EGFR signaling than seen with EGF, provoking responses in breast cancer cells associated with differentiation rather than proliferation.

Molecular Weight: 32.7 kDa. Due to glycosylation, the protein migrates to 37-40 kDa based on Bis-Tris PAGE result.

UniProt: Q61521

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation