Selectin E/CD62e Protein (AA 22-556) (His-Avi Tag)

Catalog No. ABIN7274572

This Recombinant Selectin E/CD62e protein is expressed in HEK-293 Cells.

Quick Overview for Selectin E/CD62e Protein (AA 22-556) (His-Avi Tag) (ABIN7274572)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-556

Purification tag / Conjugate: This Selectin E/CD62e protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human E-Selectin/CD62E Protein

Sequence: Trp22-Pro556

Characteristics: Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human E-Selectin, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.,Human E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-Selectin

Background: E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Molecular Weight: 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling