Selectin E/CD62e Protein (Fc Tag)

Catalog No. ABIN7197868

Product Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This Selectin E/CD62e protein is labelled with Fc Tag.

Purpose: Recombinant Rat E-Selectin/SELE Protein (Fc Tag)(Active)

Sequence: Met1-Pro494

Characteristics: A DNA sequence encoding the rat SELE (NP_620234.1) (Met1-Pro494) was expressed with the Fc region of human IgG1 at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELE-coated plates (2 μg/mL and 100 μL/well), approximately >85% cells will adhere specifically after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-Selectin/SELE (SELE Products)

Synonyms: SELE Protein, CD62E Protein, ELAM Protein, ELAM1 Protein, ESEL Protein, LECAM2 Protein, E-selectin Protein, Elam Protein, selectin E Protein, selectin, endothelial cell Protein, SELE Protein, Sele Protein

Background:

Background: E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

Synonym: SELE,Elam-1,CD62e

Molecular Weight: 78.8 kDa

NCBI Accession: NP_620234

Pathways: Thromboxane A2 Receptor Signaling