Selectin E/CD62e Protein (AA 22-557) (His tag)

Catalog No. ABIN7274574

This Recombinant Selectin E/CD62e protein is produced in HEK-293 Cells.

Quick Overview for Selectin E/CD62e Protein (AA 22-557) (His tag) (ABIN7274574)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-557

Purification tag / Conjugate: This Selectin E/CD62e protein is labelled with His tag.

Purpose: Mouse E-Selectin/CD62E Protein

Sequence: Trp22-Pro557

Characteristics: Recombinant Mouse E-Selectin/CD62E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp22-Pro557.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Mouse E-selectin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-Selectin

Background: E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Molecular Weight: 59.6 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result.

UniProt: Q00690

Pathways: Thromboxane A2 Receptor Signaling