FAP Protein (AA 26-761) (His tag)

Catalog No. ABIN7274586

This Recombinant FAP protein is produced in HEK-293 Cells.

Quick Overview for FAP Protein (AA 26-761) (His tag) (ABIN7274586)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 26-761

Purification tag / Conjugate: This FAP protein is labelled with His tag.

Purpose: Mouse FAP Protein

Sequence: Leu26-Asp761

Characteristics: Recombinant Mouse FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp761.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse FAP, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).,Mouse FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >4500 pmol/min/μg (QC Test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP

Background: Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Molecular Weight: 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result.

UniProt: P97321

Pathways: Tube Formation