Fc epsilon RI/FCER1A Protein (AA 26-205) (His tag)

Catalog No. ABIN7274607

This Recombinant Fc epsilon RI/FCER1A protein is produced in HEK-293 Cells.

Quick Overview for Fc epsilon RI/FCER1A Protein (AA 26-205) (His tag) (ABIN7274607)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-205

Purification tag / Conjugate: This Fc epsilon RI/FCER1A protein is labelled with His tag.

Purpose: Human Fc epsilon RI alpha/FCER1a Protein

Sequence: Val26-Gln205

Characteristics: Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val26-Gln205.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human Fc epsilon RI alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IgE, His-Avi Tag with the EC50 of 23.6ng/ml determined by ELISA.,Immobilized Human Fc epsilon RI alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.,Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Alternative Name: Fc epsilon RI alpha

Background: Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.

Molecular Weight: 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.

UniProt: P12319

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process