FGF10 Protein (AA 38-208) (Biotin)

Catalog No. ABIN7274615

Recombinant FGF10 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF10 Protein (AA 38-208) (Biotin) (ABIN7274615)

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 38-208

Purification tag / Conjugate: This FGF10 protein is labelled with Biotin.

Purpose: Biotinylated Human FGF10 Protein (Primary Amine Labeling)

Sequence: Gln38-Ser208

Characteristics: Recombinant Biotinylated Human FGF10 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Gln38-Ser208.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha IIIb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200) (QC Test).,Biotinylated Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.7ng/ml.,The purity of Biotinylated Human FGF10 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Alternative Name: FGF10

Background: Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.

Molecular Weight: 19.3 kDa same as Bis-Tris PAGE result.

UniProt: O15520

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus