FGF21 Protein (AA 29-209) (mFc-Avi Tag)

Catalog No. ABIN7274635

This Recombinant FGF21 protein is expressed in HEK-293 Cells.

Quick Overview for FGF21 Protein (AA 29-209) (mFc-Avi Tag) (ABIN7274635)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-209

Purification tag / Conjugate: This FGF21 protein is labelled with mFc-Avi Tag.

Purpose: Non-biotinylated Human FGF21 Protein

Sequence: His29-Ser209

Characteristics: Recombinant Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus.It contains His29-Ser209.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).,Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA (QC Test).,Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF21

Background: Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

Molecular Weight: 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9NSA1

Pathways: RTK Signaling