FGL1 Protein (AA 64-305) (Biotin,His-Avi Tag)

Catalog No. ABIN7274643

This Recombinant FGL1 protein is produced in HEK-293 Cells.

Quick Overview for FGL1 Protein (AA 64-305) (Biotin,His-Avi Tag) (ABIN7274643)

Target: FGL1 (Fibrinogen-Like 1 (FGL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 64-305

Purification tag / Conjugate: This FGL1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human FGL1 Protein

Sequence: Asp64-Asn305

Characteristics: Recombinant Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asp64-Asn305.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human FGL1, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA.,Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGL1 (Fibrinogen-Like 1 (FGL1))

Alternative Name: FGL1

Background: Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.

Molecular Weight: 31.9 kDa same as Bis-Tris PAGE result.

UniProt: Q08830