FGL2 Protein (AA 205-439) (His-Avi Tag,DYKDDDDK Tag)

Catalog No. ABIN7274644

This Recombinant FGL2 protein is expressed in HEK-293 Cells.

Quick Overview for FGL2 Protein (AA 205-439) (His-Avi Tag,DYKDDDDK Tag) (ABIN7274644)

Target: FGL2 (Fibrinogen-Like 2 (FGL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 205-439

Purification tag / Conjugate: This FGL2 protein is labelled with His-Avi Tag,DYKDDDDK Tag.

Purpose: Non-biotinylated Human FGL2 Protein

Sequence: Val205-Pro439

Characteristics: Recombinant Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGL2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.,Human FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human FGL2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGL2 (Fibrinogen-Like 2 (FGL2))

Alternative Name: FGL2

Background: Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

Molecular Weight: 31.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q14314