FLT3LG Protein (AA 27-185) (His tag)

Catalog No. ABIN7274656

This Recombinant FLT3LG protein is expressed in HEK-293 Cells.

Quick Overview for FLT3LG Protein (AA 27-185) (His tag) (ABIN7274656)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-185

Purification tag / Conjugate: This FLT3LG protein is labelled with His tag.

Purpose: Human FLT3 Ligand Protein

Sequence: Thr27-Pro185

Characteristics: Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr27-Pro185.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).,Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml.,The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt3 Ligand

Background: Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Molecular Weight: 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.

UniProt: P49771

Pathways: RTK Signaling