FLT3LG Protein (AA 27-189) (hFc Tag)

Catalog No. ABIN7274661

This Recombinant FLT3LG protein is produced in HEK-293 Cells.

Quick Overview for FLT3LG Protein (AA 27-189) (hFc Tag) (ABIN7274661)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-189

Purification tag / Conjugate: This FLT3LG protein is labelled with hFc Tag.

Purpose: Mouse FLT3 Ligand Protein

Sequence: Gly27-Gln189

Characteristics: Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly27-Gln189.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).,Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test).,Mouse FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: FLT3 Ligand

Background: Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Molecular Weight: 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: P49772

Pathways: RTK Signaling