LGALS1/Galectin 1 Protein (AA 2-135) (hFc Tag)

Catalog No. ABIN7274695

This Recombinant LGALS1/Galectin 1 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for LGALS1/Galectin 1 Protein (AA 2-135) (hFc Tag) (ABIN7274695)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-135

Purification tag / Conjugate: This LGALS1/Galectin 1 protein is labelled with hFc Tag.

Purpose: Mouse Galectin 1 Protein

Sequence: Ala2-Glu135

Characteristics: Recombinant Mouse Galectin 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala2-Glu135.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Galectin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Galectin 1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

References

Mendoza, Ballesteros, Rendon-Correa, Tonk, Warren, Snow, Stowell, Blois, Dveksler: "Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1." in: The Journal of biological chemistry, Vol. 300, Issue 9, pp. 107638, (2024) (PubMed).

Target Details

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin 1

Background: Galectin 1(Gal-1), a β-galactoside binding mammalian lectin of 14KDa, is implicated in many signalling pathways, immune responses associated with cancer progression and immune disorders. Inhibition of human Gal-1 has been regarded as one of the potential therapeutic approaches for the treatment of cancer, as it plays a major role in tumour development and metastasis by modulating various biological functions viz. apoptosis, angiogenesis, migration, cell immune escape.

Molecular Weight: 42 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.

UniProt: P16045

Pathways: Carbohydrate Homeostasis