GFRAL Protein (AA 20-350) (His-Avi Tag)

Catalog No. ABIN7274739

Product Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Protein Type: Recombinant

Protein Characteristics: AA 20-350

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This GFRAL protein is labelled with His-Avi Tag.

Purpose: Mouse GFRAL/GFR alpha-like Protein

Sequence: Gln20-Glu350

Characteristics: Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus., It contains Gln20-Glu350.

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Alternative Name: GFRAL (GFRAL Products)

Background: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Molecular Weight: 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

UniProt: Q6SJE0-1