GFRAL Protein (AA 20-351) (His-Avi Tag,Biotin)

Catalog No. ABIN7274735

Product Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Protein Type: Recombinant

Protein Characteristics: AA 20-351

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This GFRAL protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Sequence: Gln20-Glu351

Characteristics: Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 76.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM PB, 400 mM NaCl ( pH 6.0).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Alternative Name: GFRAL (GFRAL Products)

Synonyms: RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein

Background: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Molecular Weight: 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_015304775