GFRA1 Protein (AA 25-424) (His tag)

Catalog No. ABIN7274741

Recombinant GFRA1 protein expressed in HEK-293 Cells.

Quick Overview for GFRA1 Protein (AA 25-424) (His tag) (ABIN7274741)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-424

Purification tag / Conjugate: This GFRA1 protein is labelled with His tag.

Purpose: Human GFR alpha 1 Protein

Sequence: Asp25-Ser424

Characteristics: Recombinant Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Ser424.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA.,Human GFR alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFR alpha 1

Background: Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).

Molecular Weight: 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_665736