GFRA3 Protein (AA 32-382) (His tag)

Catalog No. ABIN7274742

This Recombinant GFRA3 protein is produced in HEK-293 Cells.

Quick Overview for GFRA3 Protein (AA 32-382) (His tag) (ABIN7274742)

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-382

Purification tag / Conjugate: This GFRA3 protein is labelled with His tag.

Purpose: Human GFRA3 Protein

Sequence: Asp32-Trp382

Characteristics: Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test).,Immobilized Human GFRA3, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.,Human GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human GFRA3, His Tag on Anti-His-Biosensor can bind Human ARTN, hFc Tag with an affinity constant of 5.95 nM as determined in BLI assay (Gator® Prime).,The purity of Human GFRA3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Alternative Name: GFRA3

Background: GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.

Molecular Weight: 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

UniProt: O60609