GFRA3 Protein (AA 29-379) (His tag)
Quick Overview for GFRA3 Protein (AA 29-379) (His tag) (ABIN7274743)
Target
See all GFRA3 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 29-379
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Purification tag / Conjugate
- This GFRA3 protein is labelled with His tag.
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Purpose
- Mouse GFRA3 Protein
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Sequence
- Asn29-Arg379
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Characteristics
- Recombinant Mouse GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn29-Arg379.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).,Mouse GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse GFRA3 is greater than 95% as determined by SEC-HPLC.
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GDNF Family Receptor alpha 3 (GFRA3) (AA 32-382) (Active) protein (His tag)
GFRA3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (His tag)GFRA3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
GDNF Family Receptor alpha 3 (GFRA3) protein (Fc Tag)GFRA3 Origin: Mouse Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
GDNF Family Receptor alpha 3 (GFRA3) protein (His tag)GFRA3 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
GDNF Family Receptor alpha 3 (GFRA3) (AA 1-400) protein (GST tag)GFRA3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (hIgG-His-tag)GFRA3 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (His tag)GFRA3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
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Alternative Name
- GFRA3
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Background
- GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
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Molecular Weight
- 40.3 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result.
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UniProt
- O35118
Target
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