Galectin 3 Protein (LGALS3) (AA 2-264) (His tag)

Catalog No. ABIN7274752

This Recombinant Galectin 3 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Galectin 3 Protein (LGALS3) (AA 2-264) (His tag) (ABIN7274752)

Target: Galectin 3 (LGALS3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 2-264

Purification tag / Conjugate: This Galectin 3 protein is labelled with His tag.

Purpose: Mouse Galectin 3 Protein

Sequence: Ala2-Ile264

Characteristics: Recombinant Mouse Galectin 3 Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Ala2-Ile264.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Galectin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 300 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

References

Mendoza, Ballesteros, Rendon-Correa, Tonk, Warren, Snow, Stowell, Blois, Dveksler: "Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1." in: The Journal of biological chemistry, Vol. 300, Issue 9, pp. 107638, (2024) (PubMed).

Target Details

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3

Target Type: Chemical

Background: Galectin-3 (Gal-3) is a 30 kDa β-galactose, highly conserved and widely distributed intracellularly and extracellularly. Gal-3 has been demonstrated in recent years to be a novel inflammatory factor participating in the process of intravascular inflammation, lipid endocytosis, macrophage activation, cellular proliferation, monocyte chemotaxis, and cell adhesion.

Molecular Weight: 28.5 kDa. Due to glycosylation, the protein migrates to 43-50 kDa based on Bis-Tris PAGE result.

UniProt: P16110

Pathways: RTK Signaling