GPA33 Protein (AA 22-235) (Biotin,His-Avi Tag)

Catalog No. ABIN7274767

This Recombinant GPA33 protein is expressed in HEK-293 Cells.

Quick Overview for GPA33 Protein (AA 22-235) (Biotin,His-Avi Tag) (ABIN7274767)

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-235

Purification tag / Conjugate: This GPA33 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human GPA33/A33 Protein

Sequence: Ile22-Val235

Characteristics: Recombinant Biotinylated Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).,Biotinylated Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Alternative Name: GPA33

Background: Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

Molecular Weight: 26.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

UniProt: Q99795