Hepcidin Protein (AA 59-83) (GST tag)

Catalog No. ABIN7274793

This Recombinant Hepcidin protein is produced in Escherichia coli (E. coli).

Quick Overview for Hepcidin Protein (AA 59-83) (GST tag) (ABIN7274793)

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 59-83

Purification tag / Conjugate: This Hepcidin protein is labelled with GST tag.

Purpose: Mouse Hepcidin/HAMP Protein

Sequence: Asp59-Thr83

Characteristics: Recombinant Mouse Hepcidin/HAMP Protein is expressed from E.coli with GST tag at the N-Terminus.It contains Asp59-Thr83.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Hepcidin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, 2 mM DTT ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: Hepcidin

Background: Hepcidin, the main regulator of iron metabolism, is synthesized and released by hepatocytes in response to increased body iron concentration and inflammation. Deregulation of hepcidin expression is a common feature of genetic and acquired iron disorders: in Hereditary Hemochromatosis (HH) and iron-loading anemias low hepcidin causes iron overload, while in Iron Refractory Iron Deficiency Anemia (IRIDA) and anemia of inflammation (AI), high hepcidin levels induce iron-restricted erythropoiesis.

Molecular Weight: 29.05 kDa same as Bis-Tris PAGE result.

UniProt: Q9EQ21

Pathways: Hormone Activity, Transition Metal Ion Homeostasis