ERBB4 Protein (AA 26-651) (hFc Tag)

Catalog No. ABIN7274798

Recombinant ERBB4 protein expressed in HEK-293 Cells.

Quick Overview for ERBB4 Protein (AA 26-651) (hFc Tag) (ABIN7274798)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-651

Purification tag / Conjugate: This ERBB4 protein is labelled with hFc Tag.

Purpose: Human/Cynomolgus Her4/ErbB4 Protein

Sequence: Gln26-Pro651

Characteristics: Recombinant Human/Cynomolgus Her4/ErbB4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln26-Pro651.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Cynomolgus Her4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 94.6ng/ml determined by ELISA.,Human/Cynomolgus Her4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human/Cynomolgus Her4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: Her4

Background: ErbB4, a member of the EGF receptor family, plays a variety of roles in physiological and pathological states. Genetic studies have indicated a link between ErbB4 and type 2 diabetes and obesity. ErbB4 may play an important role in glucose homeostasis and lipogenesis. ErbB4 deficiency-related obesity and adipose tissue inflammation may contribute to the development of MetS.

Molecular Weight: 96.6 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.

UniProt: Q15303

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway