HLAG Protein (AA 25-305, Monomer) (Biotin,Peptide (RIIPRHLQL),His-Avi Tag)

Catalog No. ABIN7274816

Recombinant HLAG protein expressed in HEK-293 Cells.

Quick Overview for HLAG Protein (AA 25-305, Monomer) (Biotin,Peptide (RIIPRHLQL),His-Avi Tag) (ABIN7274816)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, Monomer

Purification tag / Conjugate: This HLAG protein is labelled with Biotin,Peptide (RIIPRHLQL),His-Avi Tag.

Purpose: Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein

Sequence: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide

Specificity: Uni-Prot: P17693-1 (HLA-G), P61769 (B2M), RIIPRHLQL

Characteristics: Recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.,Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4.). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

Molecular Weight: 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response