HLAG Protein (AA 25-305, Tetramer) (Biotin,His-Avi Tag,Peptide (RIIPRHLQL))

Catalog No. ABIN7274818

This Recombinant HLAG protein is expressed in HEK-293 Cells.

Quick Overview for HLAG Protein (AA 25-305, Tetramer) (Biotin,His-Avi Tag,Peptide (RIIPRHLQL)) (ABIN7274818)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 25-305, Tetramer

Purification tag / Conjugate: This HLAG protein is labelled with Biotin,His-Avi Tag,Peptide (RIIPRHLQL).

Purpose: Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein

Sequence: Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide

Specificity: Uni-Prot: P17693-1 (HLA-G), P61769 (B2M), RIIPRHLQL

Characteristics: Recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

Molecular Weight: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response