HLAG Protein (AA 25-305, Tetramer) (His-Avi Tag,Peptide (RIIPRHLQL))

Catalog No. ABIN7274821

This Recombinant HLAG protein is expressed in HEK-293 Cells.

Quick Overview for HLAG Protein (AA 25-305, Tetramer) (His-Avi Tag,Peptide (RIIPRHLQL)) (ABIN7274821)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, Tetramer

Purification tag / Conjugate: This HLAG protein is labelled with His-Avi Tag,Peptide (RIIPRHLQL).

Purpose: Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein

Sequence: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide

Specificity: Uni-Prot: O02948 (HLA-G), Q6V7J5 (B2M), RIIPRHLQL

Characteristics: Recombinant Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).,Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test).,Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.,The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

Molecular Weight: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: O02948

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response