HVEM Protein (AA 39-202) (His tag)

Catalog No. ABIN7274831

Recombinant HVEM protein expressed in HEK-293 Cells.

Quick Overview for HVEM Protein (AA 39-202) (His tag) (ABIN7274831)

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 39-202

Purification tag / Conjugate: This HVEM protein is labelled with His tag.

Purpose: Human HVEM/TNFRSF14 Protein

Sequence: Leu39-Val202

Characteristics: Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus, It contains Leu39-Val202.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human HVEM, His Tag at 5ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA (QC Test).,Immobilized Human HVEM, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA.,Immobilized Human HVEM, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA.,Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: HVEM

Target Type: Viral Protein

Background: Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

Molecular Weight: 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Bis-Tris PAGE result.

UniProt: Q92956

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints