ICOS Protein (AA 21-144) (hFc Tag)

Catalog No. ABIN7274842

This Recombinant ICOS protein is expressed in HEK-293 Cells.

Quick Overview for ICOS Protein (AA 21-144) (hFc Tag) (ABIN7274842)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-144

Purification tag / Conjugate: This ICOS protein is labelled with hFc Tag.

Purpose: Mouse ICOS/CD278 Protein

Sequence: Glu21-Leu144

Characteristics: Recombinant Mouse ICOS/CD278 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu21-Leu144.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA.,Mouse ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse ICOS is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Molecular Weight: 40.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.

UniProt: Q9WVS0

Pathways: Cancer Immune Checkpoints