IFNA1 Protein (AA 24-189) (hFc Tag)

Catalog No. ABIN7274851

This Recombinant IFNA1 protein is expressed in HEK-293 Cells.

Quick Overview for IFNA1 Protein (AA 24-189) (hFc Tag) (ABIN7274851)

Target: IFNA1 (Interferon, alpha 1 (IFNA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 24-189

Purification tag / Conjugate: This IFNA1 protein is labelled with hFc Tag.

Purpose: Mouse IFN alpha 1 Protein

Sequence: Cys24-Lys189

Characteristics: Recombinant Mouse IFN alpha 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys24-Lys189.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 11.96 nM as determined in SPR assay (Biacore T200).,Mouse IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IFNA1 (Interferon, alpha 1 (IFNA1))

Alternative Name: IFN alpha 1

Background: IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.

Molecular Weight: 45.88 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: P01572

Pathways: JAK-STAT Signaling, Hepatitis C