IgE Protein (AA 91-421) (His tag)
Quick Overview for IgE Protein (AA 91-421) (His tag) (ABIN7274856)
Target
See all IgE ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 91-421
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Purification tag / Conjugate
- This IgE protein is labelled with His tag.
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Purpose
- Mouse IgE Protein
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Sequence
- Val91-Ser421
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Characteristics
- Recombinant Mouse IgE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val91-Ser421.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per ug by the LAL method.
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Biological Activity Comment
- Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IgE, His Tag with the EC50 of 3.1ng/ml determined by ELISA.,Mouse IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgE is greater than 95% as determined by SEC-HPLC.
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IgE (AA 209-428) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IgE
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Target Type
- Antibody
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Background
- Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
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Molecular Weight
- 38.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
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UniProt
- P06336
Target
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