IL-18 Protein (AA 37-193) (Biotin)
Quick Overview for IL-18 Protein (AA 37-193) (Biotin) (ABIN7274881)
Target
See all IL-18 (IL18) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 37-193
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Purification tag / Conjugate
- This IL-18 protein is labelled with Biotin.
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Purpose
- Biotinylated Human IL-18 Protein (Primary Amine Labeling)
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Sequence
- Tyr37-Asp193
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Characteristics
- Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Tyr37-Asp193.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-18BP, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 24.2ng/ml determined by ELISA. See testing image for detail.
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Interleukin 18 (IL18) (AA 37-193) protein
IL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 18 (IL18) (AA 37-193) protein (His-IF2DI Tag)IL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 18 (IL18) (AA 1-193) protein (GST tag)IL18 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 18 (IL18) protein (His tag)IL18 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Interleukin 18 (IL18) protein (GST tag)IL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % as determined by reducing SDS-PAGE.
Interleukin 18 (IL18) (AA 37-197) protein (Fc Tag)IL18 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 37-197) protein (mFc Tag)IL18 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 36-192) protein (His tag)IL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Interleukin 18 (IL18) (AA 36-192) proteinIL18 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4).
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Storage
- -80 °C
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Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL-18 (IL18) (Interleukin 18 (IL18))
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Alternative Name
- IL-18
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Background
- Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
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Molecular Weight
- 18.2 kDa same as Tris-Bis PAGE result.
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UniProt
- Q14116
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target
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