IL-18 Protein (His tag)

Catalog No. ABIN7320581

Product Details

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL-18 protein is labelled with His tag.

Purpose: Recombinant Mouse Interleukin-18/IL-18 Protein (His Tag)

Sequence: Asn36-Ser192

Characteristics: Recombinant Mouse Interleukin-18 is produced by our E.coli expression system and the target gene encoding Asn36-Ser192 is expressed with a 6His tag at the N-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin-18/IL-18 (IL18 Products)

Synonyms: Igif Protein, Il-18 Protein, IL-18 Protein, IGIF Protein, ChIL-18 Protein, IL-1g Protein, IL1F4 Protein, IL18 Protein, interleukin 18 Protein, Il18 Protein, IL18 Protein

Background:

Background: Interleukin-18 (IL-18)is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63 % and 91 % amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.

Synonym: Interleukin-18,Il18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Igif

Molecular Weight: 19.7 kDa

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome