IL12 Protein (AA 23-328) (His-Avi Tag,Biotin)

Catalog No. ABIN7274899

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Protein Characteristics: AA 23-328

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-12 Protein

Sequence: Ile23-Ser328 (IL-12B) /Arg23-Ser219 (IL-12A)

Specificity: Uni-Prot: P29460 (IL-12B), P29459 (IL-12A)

Characteristics: Recombinant Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-IL-12 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (IL12 Products)

Background: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Molecular Weight: 34.7(IL-12B) kDa / 25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

UniProt: P29460

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome