IL12 Protein (AA 23-328) (His-Avi Tag,Biotin)

Catalog No. ABIN7274899

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Protein Characteristics: AA 23-328

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-12 Protein

Sequence: Ile23-Ser328 (IL-12B) /Arg23-Ser219 (IL-12A)

Specificity: Uni-Prot: P29460 (IL-12B), P29459 (IL-12A)

Characteristics: Recombinant Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-IL-12 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (IL12 Products)

Synonyms: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Background: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Molecular Weight: 34.7(IL-12B) kDa / 25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

UniProt: P29460

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome