IL-23 alpha & IL-12 beta (AA 20-189) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7274925

This Recombinant IL-23 alpha & IL-12 beta protein is produced in HEK-293 Cells.

Quick Overview for IL-23 alpha & IL-12 beta (AA 20-189) (Active) protein (Biotin,His-Avi Tag) (ABIN7274925)

Target: IL-23 alpha & IL-12 beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-189

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Biotinylated Human IL-23 alpha&IL-12 beta Protein

Sequence: Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)

Specificity: Uni-Prot: Q9NPF7 (IL-23 alpha), P29460 (IL-12 beta)

Characteristics: Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of IL-23 alpha.It contains Arg20-Pro189 (IL-23 alpha) and Ile23-Ser328 (IL-12 beta).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test).,Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.,Biotinylated Human IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-23 alpha & IL-12 beta

Background: The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.

Molecular Weight: 21.7 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result.

UniProt: Q9NPF7