IL-31 Protein (AA 27-163) (His tag)

Catalog No. ABIN7274936

Recombinant IL-31 protein expressed in HEK-293 Cells.

Quick Overview for IL-31 Protein (AA 27-163) (His tag) (ABIN7274936)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-163

Purification tag / Conjugate: This IL-31 protein is labelled with His tag.

Purpose: Cynomolgus IL-31 Protein

Sequence: Leu27-Thr163

Characteristics: Recombinant Cynomolgus IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu27-Thr163.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-31, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-31 RA, His Tag with an affinity constant of 6.83 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test).,Cynomolgus IL-31 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: IL-31

Background: Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.

Molecular Weight: 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.

UniProt: G7PJ78