IL3RA Protein (AA 19-305) (Biotin,His-Avi Tag)

Catalog No. ABIN7274944

This Recombinant IL3RA protein is expressed in HEK-293 Cells.

Quick Overview for IL3RA Protein (AA 19-305) (Biotin,His-Avi Tag) (ABIN7274944)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-305

Purification tag / Conjugate: This IL3RA protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IL-3 R alpha/CD123 Protein

Sequence: Thr19-Arg305

Characteristics: Recombinant Biotinylated Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr19-Arg305.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-3 R alpha, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.,Biotinylated Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL-3 R alpha

Background: Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

Molecular Weight: 35.99 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: P26951

Pathways: JAK-STAT Signaling