IL-3 Protein (AA 27-166) (hFc Tag)

Catalog No. ABIN7274947

This Recombinant IL-3 protein is expressed in HEK-293 Cells.

Quick Overview for IL-3 Protein (AA 27-166) (hFc Tag) (ABIN7274947)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-166

Purification tag / Conjugate: This IL-3 protein is labelled with hFc Tag.

Purpose: Mouse IL-3 Protein

Sequence: Ala27-Cys166

Characteristics: Recombinant Mouse IL-3 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala27-Cys166.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse IL-3, hFc Tag on ProA-Biosensor can bind Mouse IL-3 R alpha, His Tag with an affinity constant of 0.79 μM as determined in BLI assay (Gator® Prime).,The purity of Mouse IL-3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Background: Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Molecular Weight: 42.2 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: P01586

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy