IL-4 Protein (AA 25-153)

Catalog No. ABIN7274949

This Recombinant IL-4 protein is produced in HEK-293 Cells.

Quick Overview for IL-4 Protein (AA 25-153) (ABIN7274949)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-153

Purpose: Human IL-4 Protein

Sequence: His25-Ser153

Characteristics: Recombinant Human IL-4 Protein is expressed from HEK293 without tag.It contains His25-Ser153.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.4ng/ml determined by ELISA.,Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.35 ng/mL,Loaded Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) on ProA-Biosensor can bind Human IL-4, No Tag with an affinity constant of 0.54 nM as determined in BLI assay (Gator® Prime).,The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.

Molecular Weight: 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation