IL-5 Protein (AA 20-134) (His tag)

Catalog No. ABIN7274952

This Recombinant IL-5 protein is produced in HEK-293 Cells.

Quick Overview for IL-5 Protein (AA 20-134) (His tag) (ABIN7274952)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-134

Purification tag / Conjugate: This IL-5 protein is labelled with His tag.

Purpose: Cynomolgus IL-5 Protein

Sequence: Ile20-Ser134

Characteristics: Recombinant Cynomolgus IL-5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile20-Ser134.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA.,Cynomolgus IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5

Background: IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Molecular Weight: 14.25 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5U1E7

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour