IL-15 Protein (AA 49-162)
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- Target See all IL-15 (IL15) Proteins
- IL-15 (IL15) (Interleukin 15 (IL15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 49-162
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Human IL-15 Protein
- Sequence
- Asn49-Ser162
- Characteristics
- Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.
- Purity
- >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 0.05 EU per μg by the LAL method.
- Biological Activity Comment
- Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is 0.1 0.3 ng/mL. The specific activity of recombinant human IL15 is > 1 x 10^7 units/mg, which is calibrated against the human IL15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
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Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)
IL15 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 15 (IL15) (AA 49-162), (full length) protein (His tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-15 (IL15) (Interleukin 15 (IL15))
- Alternative Name
- IL-15 (IL15 Products)
- Background
- Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
- Molecular Weight
- 12.8 kDa same as Tris-Bis PAGE result.
- UniProt
- P40933
- Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
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