IL-15 Protein (AA 49-162)

Catalog No. ABIN7274953

This Recombinant IL-15 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-15 Protein (AA 49-162) (ABIN7274953)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC

Product Details

Protein Characteristics: AA 49-162

Purpose: Human IL-15 Protein

Sequence: Asn49-Ser162

Characteristics: Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using CTLL­2 cells. The ED50 for this effect is 0.1 ­ 0.3 ng/mL. The specific activity of recombinant human IL­15 is > 1 x 10^7 units/mg, which is calibrated against the human IL­15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL-15

Background: Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

Molecular Weight: 12.8 kDa same as Tris-Bis PAGE result.

UniProt: P40933

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process