IL-5 Protein (AA 20-134) (Biotin,His-Avi Tag)

Catalog No. ABIN7274955

This Recombinant IL-5 protein is produced in HEK-293 Cells.

Quick Overview for IL-5 Protein (AA 20-134) (Biotin,His-Avi Tag) (ABIN7274955)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-134

Purification tag / Conjugate: This IL-5 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IL-5 Protein

Sequence: Ile20-Ser134

Characteristics: Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-5, His-Avi Tag with an affinity constant of 7.57 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test).,Biotinylated Human IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5

Background: IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Molecular Weight: 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour