IL-5 Protein (AA 21-133) (His-Avi Tag)

Catalog No. ABIN7274959

This Recombinant IL-5 protein is expressed in HEK-293 Cells.

Quick Overview for IL-5 Protein (AA 21-133) (His-Avi Tag) (ABIN7274959)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 21-133

Purification tag / Conjugate: This IL-5 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Mouse IL-5 Protein

Sequence: Met21-Gly133

Characteristics: Recombinant Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Met21-Gly133.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).,Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 10 % Glycerol ( pH 7.0).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5

Background: IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Molecular Weight: 16.03 kDa. Due to glycosylation, the protein migrates to 16.03 kDa and 20-32 kDa based on Bis-Tris PAGE result.

UniProt: P04401

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour