IL-8 Protein (AA 28-99) (His tag)

Catalog No. ABIN7274974

This Recombinant IL-8 protein is expressed in HEK-293 Cells.

Quick Overview for IL-8 Protein (AA 28-99) (His tag) (ABIN7274974)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-99

Purification tag / Conjugate: This IL-8 protein is labelled with His tag.

Purpose: Human IL-8/CXCL8 Protein

Sequence: Ser28-Ser99

Characteristics: Recombinant Human IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Ser99.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC₅₀ of 13.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8

Background: Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms.

Molecular Weight: 9.5 kDa. Due to glycosylation, the protein migrates to 11-13 kDa based on Tris-Bis PAGE result.

UniProt: P10145-1

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy